| dc.contributor.advisor | Kurniatin, Popi Asri | |
| dc.contributor.advisor | Artika, I Made | |
| dc.contributor.author | Syukira, Nikma | |
| dc.date.accessioned | 2025-07-11T13:37:32Z | |
| dc.date.available | 2025-07-11T13:37:32Z | |
| dc.date.issued | 2025 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/164713 | |
| dc.description.abstract | Asam fitat merupakan senyawa antinutrien yang terkandung dalam pakan hewan, sehingga perlu dihilangkan. Fitase merupakan enzim yang dapat menghidrolisis asam fitat menjadi senyawa yang inositol dan asam fosfat. Penelitian ini bertujuan mengetahui pengaruh proses fraksinasi terhadap aktivitas enzim, serta menentukan fraksi dan kinetika enzim terbaik dari fitase A. niger IPB CC 93 0265. Metode yang digunakan pada penelitian ini yaitu produksi dan isolasi enzim, fraksinasi amonium sulfat, dialisis, penentuan kadar protein, aktivitas enzim, laju awal, kinetika dan penentuan bobot molekul dengan SDS-PAGE. Hasil dari identifikasi fraksi amonium sulfat fitase memiliki aktivitas spesifik tertinggi pada fraksi 30-60% sebesar 6,46 U/mL. Hasil perhitungan kinetika enzim menunjukkan fraksi dialisis fitase A. niger memiliki nilai Vmaks sebesar 27,174 µmol/menit dan nilai Km sebesar 0,521 µmol. Identifikasi dengan SDS-PAGE menunjukkan fitase A. niger memilki berat molekul berkisar 50 kDa. | |
| dc.description.abstract | Phytic acid is an antinutrient compound in animal feed, so it must be removed. Phytase is an enzyme that can hydrolyze phytic acid into inositol and phosphoric acid compounds. This study aims to determine the effect of the fractionation process on enzyme activity, as well as determining the best fraction and enzyme kinetics of phytase A. niger IPB CC 93 0265. The methods used in this study were enzyme production and isolation, ammonium sulfate fractionation, dialysis, determination of protein content, enzyme activity, initial rate, kinetics, and determination of molecular weight by SDS-PAGE. The results of identifying the ammonium sulfate fraction of phytase had the highest specific activity in the 30-60% fraction of 6.46 U/mL. The calculation of enzyme kinetics showed that the A. niger phytase dialysis fraction had a Vmax value of 27,174 µmol/min and a Km value of 0,521 µmol. Identification by SDS-PAGE showed that A. niger phytase has a molecular weight of around 50 kDa. | |
| dc.description.sponsorship | | |
| dc.language.iso | id | |
| dc.publisher | IPB University | id |
| dc.title | Fraksinasi dan Kinetika Fitase Aspergilus niger IPB CC 93 0265 | id |
| dc.title.alternative | | |
| dc.type | Skripsi | |
| dc.subject.keyword | phytase | id |
| dc.subject.keyword | Aspergilus niger | id |
| dc.subject.keyword | fractination | id |
| dc.subject.keyword | kinetic enzyme | id |
| dc.subject.keyword | phytic acid | id |
