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      Resistansi Isolat Escherichia coli dari Daging Soto Ayam di Kantin Kampus Dramaga IPB University

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      Date
      2025
      Author
      Pratiwi, Pahlita Anggun
      Lukman, Denny Widaya
      Prasetyaningtyas, Wahono Esthi
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      Abstract
      Soto ayam merupakan makanan khas Indonesia yang berisikan suwiran daging ayam dan bahan pelengkap lainnya. Daging ayam berisiko terjadi kontaminasi Escherichia coli (E. coli) yang resistan antibiotik sehingga dapat menurunkan tingkat efektivitas antibiotik dalam menangani suatu penyakit. Penelitian ini bertujuan mengidentifikasi resistansi antibiotik dari bakteri E. coli yang diisolasi dari daging ayam suwir soto ayam yang dijual di kantin Kampus Dramaga IPB University, Bogor. Sebanyak 43 isolat yang telah teridentifikasi E. coli digunakan untuk uji resistansi antibiotik dengan metode Kirby-Bauer disc diffusion dan mengacu pada Clinical Laboratory Standard Institute 2023. Isolat E. coli menunjukkan resistansi terhadap semua antibiotik, yaitu sebesar 86% (37/43) dengan persentase sefotaksim (60,5%), ampisilin (58,1%), sulfametoksazol-trimetoprim (46,5%), kloramfenikol (37,2%), streptomisin (37,2%), dan tetrasiklin (32,6%). Isolat E. coli juga menunjukkan resistansi tingkat intermediet pada antibiotik streptomisin (48,8%), ampisilin (9,3%), kloramfenikol (9,3%), tetrasiklin (7%), sefotaksim (7%), dan sulfametoksazol-trimetoprim (4,7%). Semua isolat bakteri masih sensitif terhadap tetrasiklin (60,5%), kloramfenikol (53,5%), sulfametoksazol-trimetoprim (48,8%), ampisilin (32,6%), sefotaksim (32,6%), dan streptomisin (14%). Isolat E. coli juga bersifat multi-drug resistance dengan prevalensi tertinggi pada 4 jenis antibiotik sebesar 30,2%. Keberadaan E. coli yang resistan antibiotik pada makanan dapat berdampak negatif pada kesehatan masyarakat.
       
      Chicken noodle soup is a typical Indonesian food containing shredded chicken and other complementary ingredients. Chicken meat is at risk of contamination with antibiotic-resistant Escherichia coli (E. coli), which can reduce the effectiveness of antibiotics in treating disease. This research aimed to identify the antibiotic resistance of E. coli isolated from shredded chicken in chicken soto sold in the canteen of the Dramaga Campus of IPB University, Bogor. A total of 43 isolates identified as E. coli were used for antibiotic resistance testing using the Kirby-Bauer disc diffusion method, referring to the Clinical Laboratory Standards Institute 2023. E. coli isolates showed resistance to all antibiotics, which was 86% (37/43) with percentages of cefotaxime (60,5%), ampicillin (58,1%), sulfamethoxazole-trimethoprim (46,5%), chloramphenicol (37,2%), streptomycin (37,2%), and tetracycline (32,6%). E. coli isolates also showed intermediate-level resistance to streptomycin (48,8%), ampicillin (9,3%), chloramphenicol (9,3%), tetracycline (7%), cefotaxime (7%), and sulfamethoxazole-trimethoprim (4,7%). All bacterial isolates were still sensitive to tetracycline (60,5%), chloramphenicol (53,5%), sulfamethoxazole-trimethoprim (48,8%), ampicillin (32,6%), cefotaxime (32,6%), and streptomycin (14%). E. coli isolates were also multi-drug resistant, with the highest prevalence on 4 types of antibiotics at 30,2%. The presence of antibiotic-resistant E. coli in food can have a negative impact on public health.
       
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      http://repository.ipb.ac.id/handle/123456789/164068
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