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      Pengaruh Metode Ekstraksi terhadap Kandungan Senyawa Aktif dan Aktivitas Antioksidan Teripang dari Labuan Bajo

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      Date
      2025
      Author
      Azka, Risya Putri Noer
      Kustiariyah
      Setyaningsih, Iriani
      Winarni, Ina
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      Abstract
      Teripang tergolong hewan invertebrata berasal dari laut yang memiliki potensi untuk dijadikan sumber biofarmaka. Teripang memiliki banyak manfaat, salah satunya adalah sebagai sumber antioksidan. Tujuan penelitian ini adalah menentukan metode ekstraksi terbaik terhadap nilai rendemen dan aktivitas antioksidan dari teripang. Tahapan penelitian meliputi ekstraksi menggunakan metode maserasi, sokletasi, dan microwave, analisis aktivitas antioksidan dan komponen aktif. Nilai persen rendemen ekstrak teripang menggunakan metode maserasi lebih tinggi dibandingkan dengan rendemen ekstrak dari metode sokletasi dan microwave yaitu sebesar 3,26% pada Bohadschia marmorata dan 2,91% pada jenis Holothuria fuscopunctata. Kedua jenis teripang mengandung senyawa fenol dan steroid/triterpenoid. Aktivitas antioksidan ekstrak teripang menunjukkan sifat yang lemah pada kedua jenis teripang. Nilai aktivitas antioksidan terbaik diperoleh dari hasil ekstraksi maserasi dengan nilai IC50 sebesar 625 µg/mL pada Bohadschia marmorata dan 897 µg/mL pada teripang jenis Holothuria fuscopunctata.
       
      Sea cucumbers are marine invertebrate with the potential to be used as a source of biopharmaceuticals. They offer many benefits, one of which is their role as a source of antioxidants. The purpose of this study is to determine the best extraction method based on the yield value and antioxidant activity of sea cucumbers. The study involved several steps, including extraction using maceration, soxhlet, and microwave methods, followed by the analysis of antioxidants activity and active components. The percentage yield of sea cucumber extract using the maceration method was higher compared to the soxhlet and microwave methods, at 3.26% for Bohadschia marmorata and 2.91% for Holothuria fuscopunctata. Both species of sea cucumbers were found to contain phenolic compounds and steroid/triterpenoids. The antioxidant activity of the sea cucumber extracts showed weak properties in both species. The best antioxidant activity was obtained through the maceration method, with IC50 value of 625 µg/mL for Bohadschia marmorata and 897 µg/mL in Holothuria fuscopunctata.
       
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      http://repository.ipb.ac.id/handle/123456789/160857
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      • UT - Aquatic Product Technology [2455]

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      Indonesia DSpace Group 
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