Hubungan Temperature Humidity Index dengan Indeks Stres dan Kualitas Semen pada Domba

Abstract

Temperature Humidity Index (THI) merupakan interaksi suhu dan kelembapan udara dan memengaruhi level kenyaman hewan, sehingga menentukan tingkat stres dan kualitas semen. Penelitian bertujuan mengetahui hubungan THI terhadap indeks stres (rasio neutrofil/limfosit) dan kualitas semen pada domba. Penelitian menggunakan 4 domba jantan dewasa dan sehat klinis. Koleksi darah dan semen dilakukan sebanyak 4 kali ulangan dengan interval 3–4 hari, dan dibedakan waktu koleksi pagi dan siang hari. Koleksi darah dilakukan dari vena jugularis menjelang koleksi semen, sedangkan koleksi semen dilakukan menggunakan vagina buatan. Sampel darah dibuat preparat ulas dengan fiksasi menggunakan metanol dan pewarnaan Giemsa 10%, lalu dievaluasi diferensial leukosit, dan dihitung rasio neutrofil/limfosit (N/L). Semen dievaluasi secara makroskopis dan mikroskopis. Hasil penelitian menunjukkan bahwa THI pagi (76,21±1,65) termasuk kategori stres ringan dan ekstrem lebih rendah daripada THI siang (82,57±1,11) yang termasuk kategori stres berat. Rasio N/L pagi hari (0,70±0,06%) nyata lebih rendah daripada rasio N/L siang (1,40±0,27%). Kualitas semen yang dikoleksi pagi hari lebih baik daripada semen koleksi siang dengan perbedaan nyata pada parameter volume, skor motilitas individu, viabilitas dan abnormalitas spermatozoa, dan perbedaan sangat nyata pada parameter motilitas progresif. Disimpulkan bahwa THI berkorelasi moderat dengan indeks stres dan berkorelasi negatif kuat dengan kualitas semen pada domba.

Temperature humidity index (THI) is the interaction of air temperature and humidity and affects animal comfort levels, thus determining stress levels and semen quality. The study aimed to determine the correlation of THI to stress index (neutrophil/lymphocyte ratio) and semen quality in ram. The study used 4 mature and clinically healthy rams. Blood and semen collection was done in 4 replicates with 3-4 days interval, and differentiated collection time in the morning and afternoon. Blood collection was done from the jugular vein ahead of semen collection, while semen collection was done using an artificial vagina. Blood samples were prepared with methanol fixation and 10% Giemsa staining, then evaluated for leukocyte differential, and neutrophil/lymphocyte (N/L) ratio was calculated. Semen was evaluated macroscopically and microscopically. The results showed that THI in the morning (76.21±1.65) was categorized as mild stress and extreme lower than THI in the afternoon (82.57±1.11) which was categorized as severe stress. N/L ratio in the morning was significantly lower than the N/L ratio in the afternoon (0.70±0.06% versus 1.40±0.27%). The quality of semen collected in the morning was better than semen collected in the afternoon with significant differences in volume, individual motility score, viability and morphological abnormality parameters, and highly significant differences in progressive motility. It was concluded that THI correlated moderate with stress indices and strongly negatively correlated with semen quality in ram.