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      Pengaruh Gula Stevia dan Sukrosa terhadap Viabilitas Ragi Roti pada Adonan Bebas Gluten

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      Date
      2024
      Author
      Zahra, Cut Azifa
      Andrianto, Dimas
      Artika, I Made
      Purwani, Endang Yuli
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      Abstract
      Adonan gluten berbasis tepung terigu dalam pembuatan roti menggunakan sukrosa mampu mempertahankan viabilitas ragi roti, tetapi penggunaan sukrosa dan tepung terigu dalam waktu yang lama dapat menyebabkan gangguan kesehatan. Tujuan dari penelitian ini yaitu menentukan pengaruh gula stevia dan sukrosa terhadap viabilitas ragi roti pada adonan bebas gluten. Uji fermentasi ragi menggunakan metode tabung fermentasi, uji viabilitas ragi menggunakan CFU dan pengamatan mikroskopik dengan pewarnaan, sementara uji volume pengembangan menggunakan perbandingan volume roti sebelum dioven dan volume roti sesudah dioven. Hasil penelitian menunjukkan bahwa jumlah koloni tertinggi pada media pertumbuhan Saccharomyces cerevisiae adalah pada formulasi A1 yaitu sebesar 1,09 x 108 ± 2,83c dengan penambahan gula sukrosa tanpa stevia pada adonan roti yang menggunakan tepung singkong pregelatinisasi. Hal ini menunjukkan bahwa koloni ragi dapat berkembang pada adonan yang lebih banyak mengandung gula sukrosa dibandingkan dengan stevia. Berdasarkan penelitian yang telah dilakukan, terdapat pengaruh yang signifikan pada penambahan gula sukrosa dan stevia terhadap viabilitas ragi pada adonan bebas gluten.
       
      Wheat flour-based gluten dough in bread making using sucrose is able to maintain the viability of bread yeast, but the use of sucrose and wheat flour for a long time may result in health problems. The purpose of this study was to determine the effect of stevia sugar and sucrose on the viability of baker's yeast in gluten-free dough. The yeast fermentation test uses the fermentation tube method, the yeast viability test uses CFU and microscopic observation by staining, while the development volume test uses the comparison of the volume of bread before baking and the volume of bread after baking. The results showed that the number of colonies on Saccharomyces cerevisiae growth media that could live the most was in formulation A1, which was 1,09 x 108 ± 2,83c with the addition of sucrose sugar without stevia in bread dough using pregelatinized cassava flour. This shows that yeast colonies can develop in dough that contains more sucrose sugar than stevia. Based on the research that has been done, there is a significant effect on the addition of sucrose and stevia sugar on yeast viability in gluten-free dough.
       
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      http://repository.ipb.ac.id/handle/123456789/157412
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      • UT - Biochemistry [1463]

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