Kapasitas Antioksidan dan Inhibisi Matriks Metalloproteinase-1 oleh Kurkuminoid dari Fraksi tert-Butanol Temulawak

Abstract

Temulawak mengandung metabolit sekunder kurkuminoid yang dapat berperan sebagai antioksidan dan berpotensi menghambat enzim matriks metalloproteinase-1 (MMP-1) secara simulasi penambatan molekul. Penelitian ini bertujuan mengukur kapasitas antioksidan dan aktivitas inhibisi MMP-1 oleh fraksi tert-butanol rimpang temulawak (Curcuma xanthorrhiza). Metode ekstraksi dilakukan dengan maserasi dengan pelarut etanol 96%. Hasil maserasi difraksinasi menggunakan ammonium sulfat 30% dan tert-butanol:aquades (1:1). Kadar kurkuminoid dianalisis menggunakan High Performance Liquid Chromatography (HPLC) dengan kadar kurkuminoid total sebesar 92,54 mg/g. Kapasitas antioksidan fraksi tert-butanol temulawak diuji menggunakan metode CUPRAC dan ABTS dengan diperoleh berturut-turut sebesar 552,74 ± 39,15 dan 529,94 ± 48,89 µmol AAE/g fraksi. Uji penghambatan MMP-1 menggunakan substrat tiopeptida menunjukkan fraksi tert-butanol temulawak dapat berperan sebagai inhibitor dengan nilai IC50 sebesar 33,12 µg/mL.

Javanese turmeric contained secondary metabolites of curcuminoids that can act as antioxidants and potentially inhibit the enzyme matrix metalloproteinase-1 (MMP-1) by simulating molecular docking. This study aimed to measure the antioxidant capacity and inhibitory activity of MMP-1 by tert-butanol fraction of Javanese turmeric rhizome (Curcuma xanthorrhiza). The extraction method was carried out by maceration with 96% ethanol solvent. The results of maceration were liquid:liquid fractionation using 30% ammonium sulfate and tert-butanol:distilled water (1:1). Curcuminoid levels were analyzed using High Performance Liquid Chromatography (HPLC), resulting the total curcuminoid content was 92.54 mg/g. The antioxidant capacity of the temulawak tert-butanol fraction was tested using the CUPRAC and ABTS methods resulting 552.74 ± 39.15 and 529.94 ± 48.89 µmol AAE/g fraction. Matrix metalloproteinase-1 inhibition test using thiopeptide substrate showed temulawak tert-butanol fraction can act as an inhibitor with an IC50 value of 33.12 µg/mL.