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      Stabilitas Larutan Kerja pada Penetapan Laktat menggunakan Kromatografi Cair Kinerja Tinggi (KCKT)

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      Date
      2024
      Author
      Eryaputri, Nyi Raden Alyaa Shofaa
      Arif, Zulhan
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      Abstract
      Stabilitas merupakan kemampuan dari suatu produk untuk dapat mempertahankan sifat dan karakteristiknya yang sama seperti saat pertama reagen tersebut dibuat hingga batas tertentu. Penelitian ini dilakukan untuk menentukan kestabilan larutan kerja pada penetapan laktat, yaitu larutan uji kesesuaian sistem, fase gerak, dan larutan standar natrium laktat menggunakan kromatografi cair kinerja tinggi selama 14 hari. Hasil penelitian menunjukkan bahwa larutan uji kesesuaian sistem dan fase gerak stabil selama 14 hari sedangkan larutan standar natrium laktat stabil selama 7 hari dengan memenuhi syarat pengujian yang mengacu pada Farmakope Indonesia Edisi VI meliputi resolusi antara peak asetat dan laktat = 2% RSD waktu retensi laktat = 2%, dan tailing factor = 2. Kemudian, penentuan stabilitas larutan standar natrium laktat mencakup % RSD dari larutan standar tunggal natrium laktat = 2%, hasil individu kadar sampel laktat maupun rerata hasil kadar laktat dalam mmol/L ialah 25,43-28,11 mmol/L dengan % RSD kadar sampel laktat (standar sebagai sampel) = 2% dan % recovery 99,5-100,5%.
       
      Stability is the ability of a product to maintain the same properties and characteristics as when the reagent was first made to a certain extent. This research was conducted to determine the stability of the working solution for determining lactate, namely the system suitability test solution, mobile phase, and standard sodium lactate solution using high performance liquid chromatography for 14 days. The results showed that the system suitability test solution and mobile phase were stable for 14 days while the standard sodium lactate solution was stable for 7 days and met the test requirements referred to in the Indonesian Pharmacopoeia Edition VI, including resolution between acetate and lactate peaks = 2% RSD of lactate retention time = 2%, and tailing factor = 2. Then, determining the stability of the standard sodium lactate solution includes the % RSD of the single standard solution of sodium lactate = 2%, the individual results of the lactate sample levels and the average results of the lactate levels in mmol/L are 25.43-28.11 mmol/L with % RSD of lactate sample levels (standard as sample) = 2% and % recovery 99.5-100.5%.
       
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      http://repository.ipb.ac.id/handle/123456789/156432
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      • UT - Chemistry Analysis [48]

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