dc.contributor.advisor | Nurulita, Sari | |
dc.contributor.advisor | Hendrastuti, Elisabeth Sri | |
dc.contributor.author | Puspitaningtyas, Khasanah | |
dc.date.accessioned | 2024-07-29T06:41:04Z | |
dc.date.available | 2024-07-29T06:41:04Z | |
dc.date.issued | 2024 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/154991 | |
dc.description.abstract | Citrus is one of the economically important horticultural commodities in
Indonesia, but its national production is still not optimal. Tristeza disease caused by
Citrus tristeza virus (genus Closterovirus family Closteroviridae) is an important
disease in citrus plants and has the potential to cause significant yield losses. This
study was conducted to identify and determine the spread of tristeza disease in
several citrus-growing areas in West Java Province. The study began with field
surveys to citrus farms in four regencies, i.e. Bogor, Bandung, West Bandung, and
Garut. Chlorosis symptoms on the farm were observed to determine disease
intensity. Samples were then collected from symptomatic and asymptomatic leaves
for serological detection by double antibody sandwich enzyme-linked
immunosorbent assay (DAS-ELISA) method. Further confirmation through
molecular detection was carried out by one-step reverse transcription polymerase
chain reaction (one-step RT-PCR) method using CTV-specific primers, i.e. RBP
23-F/RBP 23-R. Field observations showed the variations in citrus cropping
conditions and varieties at each observation site. Specific symptoms of CTV
infection found involved vein clearing and chlorotic spot, leaf cupping, vein corking,
and chlorosis. Disease intensity in the field was classified as moderate to very
severe. DAS-ELISA and RT-PCR detection confirmed the distribution of the virus
in citrus growing centers in West Java except at KJ Dayat Farm in Bandung.
Nucleotide sequence analysis showed that CTV isolates from West Java consist of
four virus variants, namely T68, VT, HA16-5, and T30. Further research is needed
to determine the distribution area of each variant in Indonesia. | |
dc.description.abstract | Jeruk merupakan komoditas hortikultura penting secara ekonomi di
Indonesia, namun produksi nasional masih belum optimal. Penyakit tristeza yang
disebabkan oleh Citrus tristeza virus (genus Closterovirus famili Closteroviridae)
merupakan salah satu penyakit penting pada tanaman jeruk dan berpotensi
menyebabkan kehilangan hasil yang besar. Penelitian ini dilakukan untuk
mengidentifikasi dan mengetahui penyebaran penyakit tristeza di beberapa daerah
penanaman jeruk di Provinsi Jawa Barat. Penelitian diawali dengan survei
pengambilan sampel tanaman jeruk di empat kabupaten, yaitu Bogor, Bandung,
Bandung Barat, dan Garut. Gejala klorosis di lapangan diamati untuk menentukan
intensitas penyakit. Sampel kemudian dikumpulkan dari daun yang bergejala dan
tidak bergejala untuk deteksi virus secara serologi menggunakan metode double
antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA).
Konfirmasi lebih lanjut menggunakan deteksi molekuler dengan metode one-step
reverse transcription polymerase chain reaction (one-step RT-PCR) menggunakan
primer spesifik CTV, yaitu RBP 23-F/RBP 23-R. Pengamatan di lapangan
menunjukkan adanya variasi kondisi pertanaman dan varietas jeruk. Gejala spesifik
infeksi CTV menunjukkan vein clearing dan chlorotic spot, leaf cupping, vein
corking dan klorosis. Intensitas penyakit di lapangan tergolong sedang hingga
sangat berat. Deteksi dengan DAS-ELISA dan RT-PCR menunjukkan terdapat
infeksi CTV di seluruh lokasi pengamatan kecuali di Kebun KJ Dayat asal Bandung.
Analisis sekuen nukleotida menunjukkan bahwa isolat CTV asal Jawa Barat
memiliki empat varian virus yaitu T68, VT, HA16-5, dan T30. Perlu penelitian
lebih lanjut untuk mengetahui daerah sebar masing-masing varian di Indonesia. | |
dc.description.sponsorship | | |
dc.language.iso | id | |
dc.publisher | IPB University | id |
dc.title | Incidence and Detection of Citrus tristeza virus Infecting Citrus in West Java | id |
dc.title.alternative | Insidensi dan Deteksi Citrus tristeza virus yang Menginfeksi Tanaman Jeruk di Jawa Barat | |
dc.type | Skripsi | |
dc.subject.keyword | RT-PCR | id |
dc.subject.keyword | DAS-ELISA | id |
dc.subject.keyword | sequence analysis | id |
dc.subject.keyword | specific primers | id |
dc.subject.keyword | virus variant | id |
dc.subject.keyword | analisis sekuens | id |
dc.subject.keyword | primer spesifik | id |
dc.subject.keyword | varian virus | id |