Sifat Fisik dan Profil Protein pada Dadih Tradisional Komersil yang Diproduksi dalam Dua Bambu Berbeda
Date
2024Author
Kusuma, Ardhian Rama
Budiman, Cahyo
Arief, Irma Isnafia
Metadata
Show full item recordAbstract
Susu kerbau digunakan sebagai bahan baku dan difermentasi secara
tradisional dalam bambu. Penelitian ini meneliti karakteristik fisik dan profil
protein dadih komersial yang diproduksi dengan bambu berukuran berbeda di
Sumatera Barat. Data yang diamati meliputi pH, viskositas, kadar protein, dan SDSPAGE, yang dianalisis dengan One-Way ANOVA. Hasil penelitian menunjukkan
bahwa pH dan viskositas dadih tidak berbeda signifikan (P>0,05) antara bambu
kecil dan bambu besar. Namun, kadar protein menunjukkan perbedaan signifikan
(P<0,05). Profil protein dadih dari tabung besar menunjukkan berat molekul antara
8,14 kDa - 22,30 kDa, sedangkan dari tabung kecil berkisar antara 9,29 kDa - 57,22
kDa. SDS-PAGE mengungkapkan lima pita protein dalam dadih tabung kecil,
termasuk BSA, a-kasein, ß-laktoglobulin, dan a-laktalbumin, dengan satu pita tidak
teridentifikasi. BSA dan ß-laktoglobulin tidak ditemukan dalam dadih tabung besar,
menunjukkan laju proteolitik yang lebih ekstrim dalam tabung besar dibandingkan
tabung kecil. Buffalo milk is used as the raw material and is traditionally fermented in
bamboo. This study examines the physical characteristics and protein profiles of
commercial dadih produced using different sizes of bamboo in West Sumatra. The
observed data include pH, viscosity, protein content, and SDS-PAGE, analyzed
using One-Way ANOVA. The results of the study show that the pH and viscosity
of the dadih did not differ significantly (P>0.05) between small and large bamboo.
However, the protein content showed a significant difference (P<0.05). The protein
profile of dadih from large bamboo tubes showed molecular weights ranging from
8.14 kDa to 22.30 kDa, while in small bamboo tubes, the molecular weights ranged
from 9.29 kDa to 57.22 kDa. SDS-PAGE revealed five protein bands in dadih from
small bamboo tubes, including BSA, a-casein, ß-lactoglobulin, and a-lactalbumin,
with one band unidentified. BSA and ß-lactoglobulin were not found in dadih from
large bamboo tubes, indicating a more extreme proteolytic rate in large tubes
compared to small tubes.