Effectiveness of Clove Leaves (Syzygium aromaticum) Infusion As Antidiarrheal in Mice (Mus musculus)

Abstract

Diare adalah penyakit gastrointestinal yang umum terjadi pada berbagai spesies hewan, dengan implikasi yang signifikan terhadap kesehatan dan produktivitas hewan. Penelitian ini menyelidiki khasiat infusa daun cengkeh sebagai agen antidiare pada mencit (Mus musculus). Sebanyak 30 ekor mencit dibagi menjadi lima kelompok, yaitu kontrol negatif, kontrol positif, konsentrasi infusa daun cengkeh 100%, 50%, dan 25%. Tahapan penelitian meliputi skrining fitokimia dan uji antidiare dengan methode proteksi intestinal. Mencit diberikan berbagai perlakuan secara oral berdasarkan pembagian kelompok pada metode proteksi usus dan dipantau selama 9 jam. Parameter yang digunakan dalam metode proteksi intestinal yakni frekuensi defekasi, konsistensi feses, awal mula dan durasi diare. Analisis fitokimia menunjukkan adanya kandungan alkaloid, flavonoid, saponin, dan tanin pada ekstrak daun cengkeh. Uji ANOVA satu arah dan uji Tukey digunakan untuk menganalisis data. Hasil penelitian menunjukkan konsentrasi infusa daun cengkeh 25% dan 50% tidak efektif, sedangkan konsentrasi infusa daun cengkih 100% efektif sebagai antidiare pada mencit. Konsentrasi infusa daun cengkeh 100% terbukti efektif sebagai antidiare.

Diarrhea is a prevalent gastrointestinal ailment affecting various animal species, with significant implications for animal health and productivity. This study investigates the efficacy of Clove leaves infusion as an antidiarrheal agent in mice (Mus musculus). A total of 30 mice were divided into five groups: negative control, positive control, 100%, 50%, and 25% concentration of Clove leaves infusion. Intestinal protection methods and phytochemical screening test are used. Mice were orally administered various treatments based on group divisions in the intestinal protection method and monitored for 9 hours. The parameters used in the intestinal protection method are defecation frequency, stool consistency, and the onset and duration of diarrhea. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, saponins, and tannins in the Clove leaves extract. ANOVA one-way test and Tukey tests were used to analyze the data. The result showed that 25% and 50% concentration of Clove leaves infusion are not effective while 100% concentration of Clove leaves infusion is effective as antidiarrheal in the mice. 100% concentration of Clove leaves infusion has show the effectiveness as an antidiarrheal.