| dc.description.abstract | Pineapple is belong to the Bromeliaceae, a large family of American tropics (only
one species originated from Africa) which has 45 genera and 2 000 species. Out of
six species of pineapple, Ananas comosus (L.) Merr. is the only species grown
commercially for its fruit (Nakasone and Paull, 1998). To date, world cultivation
just based on six cultivars, i.e., Cayenne, Queen, Singapore Spanish, Red Spanish,
Abacaxi and Perolera, while the first three have been the genetic base of
distribution from the original area. In Indonesia, cultivars that recommended by
Department of Agriculture are only Queen (Bogor pineapple and Palembang
pineapple) for fresh fruit and Cayenne (Smooth or Lisse) for processing uses and
assumed to have low genetic variability. In this study, 17 accessions of pineapple
(Ananas comosus (L.) Merr.) were evaluated for their morphological performance
and subjected to analysis of Random Amplified Polymorphic DNA (RAPD).
Eight morphological characters of the accessions were measured to examine their
morphological relationship. For RAPD analysis, thirteen primers of 10-mers were
used to amplify the genomic DNA of the pineapple accessions. Out of them, three
random primers yielded monomorphic products while the remaining amplified a
total of 20 (87%) fragments, all of which were polymorphic, with an average of
1.77 fragments per primer. A dendogram based on UPGMA-link method and
principal component analysis (PCA) plots were examined the clustering pattern
and distribution of the accessions, both for morphological characters and
amplification products. As the result, RAPD analysis showed higher coefficient of
similarity between pineapple accessions tested rather than morphological's, which
may indicate the high environmental influence on the morphological performance.
This study was demonstrated that RAPD analysis was able to detect genetic
similarity among accessions in one species, eventhough among all primers used,
there was no single primer could distinguish between Queen and Smooth Cayenne
group of pineapple tested. ... | id |
