Histopatologi Limpa Macaca fascicularis dengan Infeksi Klebsiella pneumoniae
Date
2023-11-16Author
Rahmat, Nur Nadhirah
Laila, Sri Rahmatul
Pamungkas, Joko
Metadata
Show full item recordAbstract
Satwa primata pada penangkaran rentan terhadap stress dan infeksi
agen penyakit, termasuk Klebsiella sp. Keberadaan Klebsiella spp. pada
satwa ini telah dikaitkan dengan berbagai kondisi kesehatan serius,
termasuk pneumonia, meningitis, peritonitis, sistitis, dan septicemia hingga
bisa menimbulkan kematian. Dalam penelitian ini, telah dikonfirmasi bahwa
tiga individu Macaca fascicularis (1 jantan, 2 betina) di Pusat Studi Satwa
Primata (PSSP) yang mati terinfeksi oleh Klebsiella pneumoniae. Organ
limpa diambil untuk dibuat preparat histopatologi dengan pewarnaan
hematoksilin eosin dan dievaluasi lesio yang terjadi secara mikroskopis.
Hasil patologi anatomi dari limpa menunjukkan adanya lesion nodul putih
berukuran 0.5-1.0cm yang berisi massa berwarna kuning, histopatologi
menunjukkan adanya koagulasi nekrosis di pulpa putih, infiltrasi sel radang
(neutrofil, limfosit, dan sel plasma) dan hemosiderosis di pulpa putih dan
pulpa merah, serta perdarahan pada limpa di pulpa merah. Lesio yang
ditemukan menunjukkan terjadinya splenitis pada limpa. Keberadaan lesiolesio
ini akan menyebabkan disfungsi dari limpa. Primate animals are like humans in both anatomy and behavior and
are therefore widely used in biomedical research. In captivity, primate
animals are susceptible to stress and infection with disease agents, including
Klebsiella sp. The presence of Klebsiella spp. in these animals has been
associated with a variety of serious health conditions, including pneumonia,
meningitis, peritonitis, cystitis, and septicemia, that can lead to death. In this
study, it was confirmed that three Macaca fascicularis individuals (1 male
and 2 females) at the Primate Research Center (PSSP) that died were
infected by Klebsiella pneumoniae. Spleen organs were taken for
histopathological preparations with hematoxylin and eosin staining, and the
lesions were evaluated microscopically. Gross anatomy of the spleen
showed white nodules measuring 0.5-1.0 cm in diameter, filled with a
yellow, solid material. Histopathology results revealed coagulation necrosis
in the white pulp, inflammatory cell infiltration (neutrophils, lymphocytes,
and plasma cells) along with hemosiderosis in both red and white pulps, as
well as hemorrhage in the red pulp. These lesions indicate the presence of
splenitis in the spleen, leading to dysfunction in this organ.