| dc.contributor.advisor | Samosir, Agustinus Mangaratua | |
| dc.contributor.advisor | Mashar, Ali | |
| dc.contributor.author | Kurniawan, Cecep | |
| dc.date.accessioned | 2023-08-08T09:07:28Z | |
| dc.date.available | 2023-08-08T09:07:28Z | |
| dc.date.issued | 2023-08-08 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/123261 | |
| dc.description.abstract | Tachypleus gigas merupakan salah satu spesies mimi yang banyak
ditemukan di Indonesia, namun belum memiliki ketersediaan informasi biologi
molekuler yang mencukupi dengan status konservasi data deficient menurut IUCN
Red List of Threatened Species. Kajian keragaman haplotipe merupakan langkah
awal dalam upaya konservasi dan pengelolaan mimi T. gigas. Penelitian ini
bertujuan menduga kesehatan populasi mimi T. gigas di perairan Jaring Halus,
Kabupaten Langkat, Tambak Lorok, Kota Semarang, serta perairan Kampung Gisi,
Kabupaten Bintan melalui analisis keragaman haplotipe berdasarkan marka gen
16S rRNA. DNA total hasil isolasi dan ekstraksi diamplifikasi kemudian
disekuensing untuk mendapatkan urutan basa nukleotida. Hasil BLASTn
menunjukkan bahwa sampel tervalidasi Tachypleus gigas. Hasil jarak genetik mimi
T. gigas antarlokasi menunjukkan hubungan kekerabatan yang dekat. Kesehatan
populasi mimi T. gigas di Langkat dan Semarang terindikasi baik dengan nilai
keragaman haplotipe yang tinggi, sedangkan di Bintan terindikasi kurang baik
dengan nilai keragaman haplotipe yang rendah. | id |
| dc.description.abstract | Tachypleus gigas is one of horseshoe crab species found in Indonesia, but
does not yet have sufficient molecular biology information available with status
conservation data deficient according to the IUCN Red List of Threatened Species.
The study of haplotype diversity is the first step in the conservation and
management of horseshoe crab T. gigas. This study aims to predict the populations
health of T. gigas in the waters of Jaring Halus, Langkat Regency, Tambak Lorok,
Semarang City, and the waters of Gisi Village, Bintan Regency through analysis of
haplotype diversity based on 16S rRNA gene markers. Total DNA isolated and
extracted was amplified and then sequenced to obtain the nucleotide base sequence.
BLASTn results show that the sample validated by Tachypleus gigas. The results
of horseshoe crab T. gigas genetic distance between locations showed a close
kinship relationship. The population health of the horseshoe crab T. gigas in
Langkat and Semarang indicated good with high haplotype diversity values,
meanwhile in Bintan indicated poor health with low haplotype diversity value | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Keragaman Haplotipe Mimi (Tachypleus gigas, Muller 1785) berdasarkan Marka Gen 16S rRNA sebagai Dasar Konservasi | id |
| dc.title.alternative | Haplotype Diversity of Horseshoe Crab (Tachypleus gigas, Muller 1785) based on 16S rRNA Gene Markers as a Basis for Conservation | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | 16S rRNA | id |
| dc.subject.keyword | haplotype diversity | id |
| dc.subject.keyword | Tachypleus gigas | id |
