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      Aktivitas Antibakteri dan Identifikasi Senyawa Bioaktif dari Ekstrak Daun Mangkokan (Polyscias scutellaria)

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      Date
      2023
      Author
      Ekaviolita, Erika
      Tjahjoleksono, Aris
      Wahyudi, Aris Tri
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      Abstract
      Tanaman mangkokan (Polyscias scutellaria) merupakan salah satu tanaman obat Indonesia yang biasanya dimanfaatkan sebagai obat tradisional, tanaman hias, tanaman pagar, dan sumber pangan. Ekstrak daun mangkokan mengandung senyawa dengan aktivitas antibakteri. Penelitian ini bertujuan menguji aktivitas antibakteri ekstrak daun mangkokan, serta mengidentifikasi senyawa bioaktif yang terkandung dalam ekstrak. Ekstraksi daun mangkokan dilakukan menggunakan pelarut metanol, etanol, etil asetat, dan n-heksana. Ekstrak diuji aktivitas antibakteri dan diidentifikasi senyawa bioaktifnya menggunakan GC-MS. Hasil uji antibakteri menunjukkan bahwa ekstrak metanol dan etanol memiliki aktivitas antibakteri terhadap Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, dan Bacillus subtilis. Ekstrak n-heksana hanya memiliki aktivitas antibakteri terhadap E. coli, sedangkan ekstrak etil asetat tidak menunjukkan aktivitas antibakteri. Berdasarkan hasil analisis GC-MS, semua ekstrak daun mangkokan mengandung senyawa bioaktif dengan aktivitas antibakteri. Penelitian lebih lanjut diperlukan untuk menampilkan potensi antibakteri ekstrak daun mangkokan secara lebih baik.
       
      The mangkokan plant (Polyscias scutellaria) is one of the Indonesian medicinal plants which are usually used as a traditional medicine, ornamental plant, hedge plant, and food source. Mangkokan leaf extract contain antibacterial activity compounds. This study aims to examine the antibacterial activity of mangkokan leaf extract, as well as to identify the bioactive compounds contained in the extract. Mangkokan leaf extraction was carried out using methanol, ethanol, ethyl acetate, and n-hexane solvents. The extracts were tested for antibacterial activity and the bioactive compounds were identified using GC-MS. The results of the antibacterial test showed that the methanol and ethanol extracts had antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus subtilis. The n-hexane extract only had antibacterial activity against E. coli, while the ethyl acetate extract did not show antibacterial activity. Based on the results of GC-MS analysis, all mangkokan leaf extracts contained bioactive compounds which have antibacterial activities. Further research is needed to reveal the antibacterial potential of mangkokan leaf extract.
       
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      http://repository.ipb.ac.id/handle/123456789/116428
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