dc.contributor.advisor | Sukenda, Sukenda | |
dc.contributor.advisor | Yuhana, Munti | |
dc.contributor.author | Sitanggang, Bertha Citra Asih Br. | |
dc.date.accessioned | 2022-04-22T05:48:17Z | |
dc.date.available | 2022-04-22T05:48:17Z | |
dc.date.issued | 2022-04-22 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/111651 | |
dc.description.abstract | Ikan nila (Oreochromis niloticus) merupakan salah satu komoditas air tawar
unggulan yang mengalami peningkatan produksi, namun masih belum memenuhi
target produksi setiap tahun. Hal tersebut disebabkan oleh penyakit streptococcosis
di pembesaran ikan nila yang penyebabnya adalah Streptococcus agalactiae.
Vaksinasi adalah salah satu upaya penanggulangan penyakit ikan yang dapat
diaplikasikan secara oral melalui pakan. Penelitian ini bertujuan menguji efikasi
vaksin oral Streptococcus agalactiae pada ikan nila dengan penerapan dosis yang
berbeda serta menganalisis sistem imun spesifik dan nonspesifik ikan nila yang
divaksinasi. Ikan nila yang digunakan memiliki bobot awal 10,72 ± 0,95 g dan
panjang 8,72 ± 0,44 cm. Pemeliharaan berlangsung selama 30 hari dan uji tantang
selama 14 hari dengan bakteri S. agalactiae. Perlakuan yang diterapkan meliputi
kontrol negatif (K-), kontrol positif (K+), 105 CFU/g pakan (A), 107 CFU/g pakan
(B) dan 109 CFU/g pakan (C). Setelah vaksinasi, ikan dipelihara selama 30 hari dan
diberi pakan komersial secara at satiation. Hasil penelitian menunjukan aplikasi
vaksin oral mampu meningkatkan kelangsungan hidup relatif, serta respons imun
berbeda nyata (P<0,05) dibandingkan kontrol. Vaksinasi dengan dosis 107 CFU/g
pakan sudah dapat memberi proteksi terhadap serangan S. agalactiae dan
meningkatkan kekebalan spesifik yang terlihat pada peningkatan level antibodi. | id |
dc.description.abstract | Tilapia (Oreochromis niloticus) is one of the leading freshwater
commodities which has increased production, but it still does not meet the annual
production target. This is caused by streptococcosis disease which is the cause is
Streptococcus agalactiae in the grow-out of tilapia. Vaccination is one of the efforts
to control fish diseases that can be applied orally through the feed. This study aimed
to test the efficacy of Streptococcus agalactiae oral vaccine in tilapia with different
doses applied and analyze the specific and non-specific immune systems of
vaccinated tilapia. The tilapia used had an initial weight of 10,72 ± 0,95 g and a
length of 8,72 ± 0,44 cm. Maintenance lasted for 30 days and trials for 14 days with
S. agalactiae. The treatments applied included a negative control (K-), positive
control (K+), 105 CFU/g feed (A), 107 CFU/g feed (B), and 109 CFU/g feed (C).
After vaccination, fish were reared for 30 days and fed commercial feed on at
satiation. The results showed that oral vaccine application was able to increase
relative survival and immune response and was significantly different (P<0,05)
compared to controls. Vaccination with a dose of 107 CFU/g of feed was able to
provide protection against S. agalactiae attacks and increase specific immunity as
seen in the increase in antibody levels. | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Efikasi Vaksin Oral Streptococcus agalactiae dengan Dosis yang Berbeda pada Ikan Nila (Oreochromis niloticus) | id |
dc.title.alternative | Efficacy of Streptococcus agalactiae Oral Vaccine with Different Doses in Tilapia (Oreochromis niloticus) | id |
dc.type | Undergraduate Thesis | id |
dc.subject.keyword | dose | id |
dc.subject.keyword | tilapia | id |
dc.subject.keyword | protection | id |
dc.subject.keyword | Streptococcus agalactiae | id |
dc.subject.keyword | oral vaccine | id |