| dc.contributor.advisor | Istikorini, Yunik | |
| dc.contributor.advisor | Siregar, Ulfah | |
| dc.contributor.author | Cahyani, Nofita | |
| dc.date.accessioned | 2021-11-16T07:14:13Z | |
| dc.date.available | 2021-11-16T07:14:13Z | |
| dc.date.issued | 2021 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/109925 | |
| dc.description.abstract | Sengon (Falcataria moluccana Miq.) merupakan tanaman kehutanan yang dalam pengelolaannya mengalami serangan patogen, diantaranya karat tumor. Pengendalian karat tumor menggunakan fungisida terbentur masalah pencemaran lingkungan dan resistensi terhadap fungisida. Karena itu perlu dilakukan pengendalian terpadu, salah satunya dengan mikoparasit. Penelitian ini bertujuan mengisolasi dan mengidentifikasi cendawan yang terdapat pada karat tumor sengon secara morfologi dan molekuler. Terdapat 12 isolat cendawan yang berhasil diisolasi dari isolat karat tumor dengan bercak hitam atau hijau keabuan di permukaannya. Isolat kemudian diidentifikasi secara makroskopis dan mikroskopis untuk melihat morfologi dan struktur cendawan. Identifikasi molekuler dilakukan dengan metode DNA barcode. Hasil identifikasi menunjukkan 12 isolat terdiri dari 9 jenis cendawan yaitu Fusarium oxysporum, F. semitectum, F. solani, F. clamydosporum, F. circinatum, F. incarnatum, F. verticilioides, Lasiodiplodia theobromae, dan Pestalotiopsis palmarum. DNA barcoding dapat mengatasi keterbatasan identifikasi morfologi sehingga identifikasi molekuler menjadi sangat mendukung identifikasi morfologi. Kata kunci: DNA barcoding, Falcataria moluccana, karat tumor, mikoparasit | id |
| dc.description.abstract | Sengon (Falcataria moluccana Miq.) plantation often severely attacked by diseases, particularly gall rust. Controlling tumor rust using fungicides could pose problems of environmental pollution and resistance to fungicides. Therefore, integrated control is required, one of which using mycoparasites. The aim of this study was to isolate and identify the fungi found on the sengon gall rust morphologically and molecularly. Fungi were isolated from the gall rust samples with black or gray-green patches on the surface, resulting in 12 isolates. The isolates were then identified macroscopically and microscopically to see the morphology and structure of the fungus. DNA barcodes were used identify the fungi species. DNA barcode identification showed 12 isolates consisted of 9 species of fungi, namely Fusarium oxysporum, F. semitectum, F. solani, F. clamydosporum, F. circinatum, F. incarnatum, F. verticilioides, Lasiodiplodia theobromae, and Pestalotiopsis palmarum. DNA barcodes has proven its ability to overcome difficulty in species identification using morphology that it’s utilization is highly recommended. Keywords: DNA barcoding, Falcataria moluccana, gall rust, mycoparasites | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Isolasi dan Identifikasi Cendawan Yang Terdapat pada Karat Tumor Sengon | id |
| dc.title.alternative | Isolation and Identification of Fungi Found on Sengon Gall Rust Disease | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | DNA barcoding | id |
| dc.subject.keyword | Falcataria moluccana | id |
| dc.subject.keyword | gall rust | id |
| dc.subject.keyword | mycoparasites | id |
