dc.contributor.advisor | Rahayuningsih, Mulyorini | |
dc.contributor.author | Rizal, Syaiful | |
dc.date.accessioned | 2021-09-27T10:16:01Z | |
dc.date.available | 2021-09-27T10:16:01Z | |
dc.date.issued | 2021 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/109415 | |
dc.description.abstract | Ganoderma boniense merupakan cendawan patogen yang menyebabkan
penyakit busuk pangkal batang (BPB) pada tanaman kelapa sawit. Penelitian ini
mengkaji suatu alternatif dalam mengendalikan G. boninense melalui pemanfaatan
agens pengendali hayati (APH) yang lebih ramah lingkungan daripada penggunaan
fungisida komersial. Seleksi bakteri endofit sebagai APH dilakukan melalui isolasi
bakteri dari akar kelapa sawit, mengukur persen zona hambatan terhadap isolat G.
boninense secara in vitro, dan uji hipersensitif pada tanaman tembakau. Isolat
bakteri endofit terpilih A2 dan C5 memiliki daya hambat terhadap G. boninense
berturut-turut sebesar 35% dan 47%. Kedua isolat tersebut teridentifikasi secara
molekuler berturut-turut sebagai bakteri Lysinibacillus xylanilyticus (99,85%) dan
bakteri Aeromonas hydrophila (99,64%). Bakteri endofit terpilih diformulasikan ke
dalam formulasi kompos, tepung, dan cair. Ketiga jenis formula dapat
mempertahankan viabilitas sel sampai dengan 106 cfu/ml serta lebih stabil dan
adaptif pada formulasi cair dengan kepadatan berkisar (8,0–9,0)×106
cfu/ml pada
akhir minggu pengamatan dibandingkan formulasi lainnya. | id |
dc.description.abstract | Ganoderma boninense is a pathogenic fungus that causes basal stem rot
(BSR) diseases in oil palm plantations. This research examined an alternative in
controlling G. boninense through the use of biological control agents (BCAs) which
was more environmentally friendly than the use of commercial fungicide.
Endophytic bacteria as BCAs were selected by isolating bacteria from oil palm
roots, measuring the percent zone of inhibition against G. boninense based on in
vitro test, and presenting hypersensitivity test on tobacco plant. Selected endophytic
bacterias namely A2 and C5 had inhibiton against G. boninense of 35% and 47%,
respectively. Those isolates were identified molecularly as Lysinibacillus
xylanilyticus (99,85%) and Aeromonas hydrophila (99,64%), respectively. The
selected endophytic bacterias were formulated in compost, flour, and liquid
formulation. Those three types of formula could maintain cell viability up to 106
cfu/ml and are more stable also adaptive in liquid formulation within a range of
(8,0–9,0)×106
cfu/ml at the end of the observation compared to other formulations. | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Isolasi, Identifikasi, dan Formulasi Media Bakteri Endofit untuk Membatasi Serangan Cendawan Ganoderma pada Kelapa Sawit | id |
dc.title.alternative | Isolation, Identification, and Media Formulation of Endophytic Bacteria to Limit Ganoderma Fungus Attacks on Oil Palm | id |
dc.type | Undergraduate Thesis | id |
dc.subject.keyword | basal stem rot (BSR) | id |
dc.subject.keyword | endophytic bacteria | id |
dc.subject.keyword | Ganoderma boninense | id |
dc.subject.keyword | media formulation | id |
dc.subject.keyword | oil palm | id |