| dc.contributor.advisor | Siregar, Iskandar Zulkarnaen | |
| dc.contributor.advisor | Pratama, Rahadian | |
| dc.contributor.author | Nurfajri, Siska | |
| dc.date.accessioned | 2021-06-07T14:17:54Z | |
| dc.date.available | 2021-06-07T14:17:54Z | |
| dc.date.issued | 2021 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/106947 | |
| dc.description.abstract | Balsa (O. pyramidale) merupakan salah satu spesies tanaman hutan cepat
tumbuh bernilai ekonomi yang diintroduksi ke Indonesia dengan informasi genetik
yang masih terbatas. Penelitian ini bertujuan untuk menghasilkan data genomik dan
menganalisis hubungan kekerabatan balsa (O. pyramidale) berbasis long-read
sequences dengan teknologi Oxford Nanopore (ONT). Penelitian terdiri dari
beberapa tahapan yaitu ekstraksi DNA, uji kualitas dan kuantitas DNA, proses
sekuensing DNA menggunakan mesin MinION ONT, dan analisis bioinformatik.
Hasil penelitian menunjukkan bahwa proses sekuensing pada balsa (O. pyramidale)
menghasilkan sekuen partial whole genome kloroplas sebesar 155.430 bp yang
dapat digunakan untuk analisis hubungan kekerabatan berbasis DNA barcode pada
genom kloroplas. Analisis filogeni menunjukkan bahwa jenis balsa (O. pyramidale)
secara genetik (multi-genotipe) berada satu clade dengan sesama jenis O.
pyramidale pada setiap penggunaan gen atau marka baik rbcL, matK, maupun
kombinasi gen rbcL dan matK. Jenis gen atau marka yang cocok digunakan dalam
analisis hubungan kekerabatan pada balsa (O. pyramidale) dapat menggunakan
matK dan kombinasi rbcL dan matK. | id |
| dc.description.abstract | Balsa (O. pyramidale) is one of the fast-growing forest plant species with high
economic value and was introduced to Indonesia, but there is not much genetic
information available. The main objective of this research was to generate genomic
data and analyze the phylogeny of balsa (O. pyramidale) based on long-read
sequences with Oxford Nanopore Technology (ONT). The research consisted of
several steps: i) DNA extraction, ii) testing the quality and quantity of DNA, iii) the
DNA sequencing process using a machine MinION ONT, and vi) bioinformatic
analysis. Research results showed that the sequencing process in balsa (O.
pyramidale) produced partial whole-genome chloroplast of 155,430 bp which can
be used for relationship analysis based on DNA barcodes on the genome. Phylogeny
analysis showed that balsa (O. pyramidale) genetically (multi-genotype) is
positioned in the same clade with other O. pyramidale deposited samples in every
use of genes or markers rbcL, matK, or a combination of genes rbcL and matK.
MatK or a combination of both rbcL and matK can be recommended to be used for
analysis phylogeny of balsa (O. pyramidale). | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Analisis Long-Read Sequences dengan Teknologi MinION Oxford Nanopore pada Balsa (Ochroma pyramidale (Cav. ex Lam.) Urb.) | id |
| dc.title.alternative | Long-Read Sequences Analysis with MinION Oxford Nanopore Technology of Balsa (Ochroma pyramidale (Cav. ex Lam.) Urb.) | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | matK | id |
| dc.subject.keyword | MinION | id |
| dc.subject.keyword | Ochroma pyramidale | id |
| dc.subject.keyword | phylogenetic | id |
| dc.subject.keyword | rbcL | id |
