| dc.contributor.advisor | Santoso, Sugeng | |
| dc.contributor.advisor | Wiyono, Suryo | |
| dc.contributor.author | Sadiah, Siti Anis Satus | |
| dc.date.accessioned | 2021-03-22T07:34:04Z | |
| dc.date.available | 2021-03-22T07:34:04Z | |
| dc.date.issued | 2020 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/106373 | |
| dc.description.abstract | Wereng cokelat adalah hama padi terpenting di Indonesia. Pengendalian
wereng cokelat dapat dikembangkan dengan berbagai metode. Cendawan endofit
berpotensi sebagai agen biokontrol serangga. Penelitian ini bertujuan mengetahui
potensi cendawan endofit dalam mengendalikan wereng cokelat. Cendawan endofit
diisolasi dari padi dan rumput-rumputan di sekitar Bogor. Sebanyak 12 isolat hasil
isolasi, 7 isolat asal padi dan 5 asal rumput-rumputan. Berdasarkan hasil uji
patogenisitas, terdapat 5 isolat adalah nonpatogen. Uji selanjutnya dengan
menggunakan 4 isolat cendawan endofit, yaitu Acremonium sp., Nigrospora sp.1,
Nigrospora sp.2, Nigrospora sp.3. Penggunaan cendawan Nigrospora sp.4 dari
koleksi Klinik Tanaman sebagai kontrol positif. Benih padi direndam ke dalam
suspensi konidia cendawan endofit selama dua jam dan diperam selama 24 jam.
Bibit padi dipindahkan ke ember setelah tanaman berumur 3 minggu setelah semai.
Bibit padi disemprot dengan suspensi cendawan endofit seminggu setelah tanam.
Tujuh hari setelah inokulasi cendawan endofit, sebanyak 10 nimfa wereng cokelat
instar satu diintroduksi ke tanaman padi dan diamati setiap hari selama 7 hari untuk
mencatat kelangsungan hidup nimfa. Dalam percobaan lain, 5 pasang wereng
cokelat dewasa diintroduksi ke tanaman padi (28 hari setelah tanam) dan diamati
setiap minggunya selama 6 minggu. Semua isolat cendawan endofit yang diuji
dalam percobaan ini menyebabkan kematian nimfa dan menurunkan populasi
wereng cokelat. | id |
| dc.description.abstract | Brown planthopper (BPH) is the most important rice pest in Indonesia. We
developed many methods to control this planthopper. Endophytic fungi have the
potential as biocontrol agents of insects. This study aims to determine the potential
of endophytic fungi in controlling BPH. Endophytic fungi were collected from rice
and grasses in Bogor. A total of 12 isolates were isolated, 7 from rice and 5 from
grasses. Based on test, 5 isolates were nonpathogenic. We used 4 of these isolates
for the experiments, Acremonium sp., Nigrospora sp.1, Nigrospora sp.2,
Nigrospora sp.3. We also used Nigrospora sp.4 from Klinik Tanaman collection as
positive control. Rice seeds were dipped in endophytic conidia fungal suspension
for two hours, and then ripened for 24 hours. Rice seedlings were transferred to the
planting baskets 3 weeks after sowing. Rice seedlings were sprayed with
endophytic fungal suspension a week after planted. Seven days after inoculation of
endophytic fungi, 10 first-stage BPH nymphs were introduced and observed daily
for 7 days to record nymphs survival. In the other experiment, 5 pairs of adult were
introduced in to the rice plants (28 days after treatment) and observed weekly for
six weeks. All of endophytic fungi isolates tested in these experiments caused
nymphs death and population suppression of BPH. | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Potensi Cendawan Endofit asal Padi dan Rumputrumputan untuk Pengendalian Wereng Cokelat Nilaparvata lugens (Stal) (Hemiptera: Delphacidae). | id |
| dc.title.alternative | Potential of Endophytic Fungi Originated for Rice and Grasses for Controling Brown planthopper Nilaparvata lugens (Stal) (Hemiptera: Delphacidae). | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | biological control | id |
| dc.subject.keyword | Nigrospora | id |
| dc.subject.keyword | population | id |
| dc.subject.keyword | resistent | id |
