| dc.contributor.author | Saputra, F. | |
| dc.contributor.author | Jakaria | |
| dc.contributor.author | Sumantri, Cece | |
| dc.date.accessioned | 2017-03-07T04:43:10Z | |
| dc.date.available | 2017-03-07T04:43:10Z | |
| dc.date.issued | 2013-12 | |
| dc.identifier.issn | 0126-0472 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/83791 | |
| dc.description.abstract | The objective of this research was to identify genetic variation of mitochondria DNA especially in cytochrome oxidase subunit I (COI) among population of Indonesian buffaloes. Samples of swamp buffaloes were collected from Aceh (n= 3), North Sumatra (n= 3), Riau (n= 3), Banten (n= 3), Central Java (n= 3), West Nusa Tenggara (n= 3) and South Sulawesi (n= 3), and riverine buffalo from North Sumatra (n= 1) out of group for comparison. Sequence of COI was analyzed using MEGA 5.10 software with neighbor-joining method kimura 2-parameter model to reconstruct phylogeny tree. The result showed that three haplotypes for swamp buffalo and one haplotype for riverine buffalo in Indonesia resulted from 41 polymorphic sites. This finding showed that the COI gene could be considered as a marker to distinguish among swamp buffaloes in Indonesia | id |
| dc.description.abstract | Tujuan penelitian ini adalah untuk mengidentifikasi keragaman DNA mitokondria, khususnya pada daerah cytochrome oxidase subunit I (COI) pada delapan populasi kerbau lokal Indonesia. Sampel kerbau lumpur berasal dari Aceh (n= 3), Sumatra Utara (n= 3), Riau (n= 3), Banten (n= 3), Jawa Tengah (n= 3), Nusa Tenggara Barat (n= 3) dan Sulawesi Selatan (n= 3); dan sebagai pembanding di-gunakan kerbau sungai yang berasal dari Sumatra Utara (n= 1). Sekuen gen COI dianalisis menggu-nakan program MEGA 5.10 software dengan metoda neighbor-joining substitusi kimura 2 parameter untuk merekonstruksi pohon filogeni. Hasil penelitian menunjukkan adanya 41 situs polymorfime dan dapat dikelompokkan kedalam tiga haplotipe untuk kerbau lumpur dan satu haplotipe untuk kerbau sungai. Gen COI dapat dipakai sebagai penciri genetik untuk membedakan antar populasi kerbau di Indonesia | id |
| dc.language.iso | en_US | id |
| dc.publisher | Media Peternakan | id |
| dc.relation.ispartofseries | 36;3 | |
| dc.title | Genetic Variation of mtDNA Cytochrome Oxidase Subunit I (COI) in Local Swamp Buffaloes in Indonesia | id |
| dc.type | Article | id |
| dc.subject.keyword | COI | id |
| dc.subject.keyword | filogeni | id |
| dc.subject.keyword | kerbau Indonesia | id |
| dc.subject.keyword | variasi genetik | id |
