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Production Of Chimeric Calves Derived From Parthenogenetic And Fertilized Bovine Embryos
(IPB (Bogor Agricultural University), 1993)
The present study was aimed to examine whether parthenogenetic cells could contribute to the development of bovine embryos to term. Chimeric blastocysts were produced by aggregation of parthenogenetic and in vitro fertilized ...
Calves Obtained After Transfer Of Frozen-Thawed Bovine Immature Oocytes Cryopreserved In Various cryoprotectants
(IPB (Bogor Agricultural University), 1993)
The objective of this study was to evaluate the fertilization and cleavage rates in vitro of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. Various permeating cryoprotective agents [1.8M ethylene glycol ...
Survival rate of frozan-thawed bovine IVF embryos in relation to exposure time using various cryoprotectans
(IPB (Bogor Agricultural University), 1993)
The objective of this study was to examine the relationship between cryoprotectant and equilibration t1me on development of IVF derived embryos in culture after thawing. Good to excellent quality day 1 to 8 blastocysts ...
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
(IPB (Bogor Agricultural University), 1993)
The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ...
Effect Of The Presence Of A Cl In The Ovary On Oocyte Number, Cleavage Rate And Blastocyst Production In Vitro In Cattle
(IPB (Bogor Agricultural University), 1993)
High progesterone concentration has been shown to cause regression of the dominant follicle and follicle wave turnover in cattle (Taylor and Rajamahendran, can J Anim Sci 74:281,1994). Pregnant cows also produced a higher ...
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
(IPB (Bogor Agricultural University), 1993)
Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ...
The Influence Of Sperm-Oocyte Incubation Time And Breed Of Bull On In Vitro Embryo Development In Catile
(IPB (Bogor Agricultural University), 1993)
It has been reported that the length of sperm-oocyte-incubation time using Holstein sperm did not affect to the embryo development (Rehman, et al. Theriogenology 41:1447- 1452, 1994). Therefore, the present study was ...
Aggregation of parthenogenetic and fertilized embryos for producing chimera calves
(IPB (Bogor Agricultural University), 1993)
Chimeric blastocysts were produced by aggregation of parthenogenetic and fertilized demi-embryos from Japanese Brown breed and Holstein breed cattle, respectively. The fertilized embryos were obtained by in vitro maturation, ...
Development Of A Simple, Portable Carbon Dioxide Incubator For Production Of Bovine Ivf Embryos
(IPB (Bogor Agricultural University), 1993)
We have developed a simple, economical and portable C02 incubator for production of bovine IVF embryos in the field. The incubator consists of a metallic chamber (29L x 26W x 21 H em); the bottom plate of this chamber is ...
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
(IPB (Bogor Agricultural University), 1993)
Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ...