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Chimera production in bovine and its sperm capacity
(Bogor Agriculture University, 1998)
In vitro produced bovine embryos were used to produce chimeras by combining the 8- cell stage embryos. Aggregated embryos were embedded in either 0, 1.0 and 1.2% agar and cultured in vitro. The aggregation ...
Perkembangan In Vitro Embrio Sapi-Sapi Holstein dan Japanese Black
(IPB (Bogor Agriculture University), 1996)
Two experiments were conducted with 3,457 oocytes, aspirated from the ovaries of slaughtered Holstein and Japanese Black breeds cows to compare the use of superovulated cow serum (SCS) and fetal calf serum (FCS) supplementation ...
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(IPB (Bogor Agricultural University), 1996)
In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...
Effects of Centrifugation and Lipid Removal on the Cryopreservation of in Vitro Produced Bovine Embryos at the Eight-Cell Stag
(IPB (Bogor Agricultural University), 1998)
The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes ...
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(IPB (Bogor Agricultural University), 1997)
The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...
Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos
(IPB (Bogor Agricultural University), 1998)
The objective of this study was to develop a simple and portable CO2 incubator using effervescent granules EG.and to examine the effect of negative and positive air pressure for in vitro maturation IVM., fertilization ...
The Influence of Polyvinypyrrolidone on Freezing of Bovine IVF Blastocysts Following Biopsy
(IPB (Bogor Agricultural University), 1996)
A Study was conducted to develop a better freezing protocol for in vitro developed biosied blastocyst. Biopsied blastocysts exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different concentration (5,10, ...
Morphological Classification of The Ovaries in Relation to The Subsequent oocyte Quality for IVF-Produced Bovine Embryos
(IPB (Bogor Agricultural University), 1998)
Although some inferences have been made regarding the morphological aspects of the ovades in relation to the subsequent oocyte developmental competence in an in vitro system, the influence of ovarian morphology, taken as ...
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(IPB (Bogor Agricultural University), 1996)
The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ...
Viability Of Bovine Blastocysts Obtained After 7, 8 Or 9 Days Of Culture Following Vitrification And One-Step Rehydration
(IPB (Bogor Agricultural University), 1993)
This study examined the morphological appearance, hatching rates and live:dead cell ratios following vitrification of in vitro produced (IVP) bovine blastocysts. Expanded blastocysts obtained after 7, 8 or 9 d of culture ...