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Survival rate of frozan-thawed bovine IVF embryos in relation to exposure time using various cryoprotectans
(IPB (Bogor Agricultural University), 1993)
The objective of this study was to examine the relationship between cryoprotectant and equilibration t1me on development of IVF derived embryos in culture after thawing. Good to excellent quality day 1 to 8 blastocysts ...
The Role Of Pre-Equilibration For Vitrification Of In Vitro Produced (Ivp) Bovine Embryos
(IPB (Bogor Agricultural University), 1993)
The role of equilibration for successful vitrification was investigated by one-step or three-step addition ard one-step removal of the cryoprotective agents (CPAs) in vitrification solution. The blastocysts ard expandedblastocysts ...
Effect Of The Presence Of A Cl In The Ovary On Oocyte Number, Cleavage Rate And Blastocyst Production In Vitro In Cattle
(IPB (Bogor Agricultural University), 1993)
High progesterone concentration has been shown to cause regression of the dominant follicle and follicle wave turnover in cattle (Taylor and Rajamahendran, can J Anim Sci 74:281,1994). Pregnant cows also produced a higher ...
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
(IPB (Bogor Agricultural University), 1993)
Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ...
The Influence Of Sperm-Oocyte Incubation Time And Breed Of Bull On In Vitro Embryo Development In Catile
(IPB (Bogor Agricultural University), 1993)
It has been reported that the length of sperm-oocyte-incubation time using Holstein sperm did not affect to the embryo development (Rehman, et al. Theriogenology 41:1447- 1452, 1994). Therefore, the present study was ...
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
(IPB (Bogor Agricultural University), 1993)
Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ...
Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
(IPB (Bogor Agricultural University), 1993)
Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ...
Viability Of Bovine Blastocysts Obtained After 7, 8 Or 9 Days Of Culture Following Vitrification And One-Step Rehydration
(IPB (Bogor Agricultural University), 1993)
This study examined the morphological appearance, hatching rates and live:dead cell ratios following vitrification of in vitro produced (IVP) bovine blastocysts. Expanded blastocysts obtained after 7, 8 or 9 d of culture ...