Browsing Articles by Author "M. Takagi"
Now showing items 1-7 of 7
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Calves Obtained After Transfer Of Frozen-Thawed Bovine Immature Oocytes Cryopreserved In Various cryoprotectants
Suzuki, Tatsuyuki | M. Takagi | Boediono, Arief (1993)The objective of this study was to evaluate the fertilization and cleavage rates in vitro of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. Various permeating cryoprotective agents [1.8M ethylene glycol ... -
Chimeric Blastocyst Production Using Ivf Bovine Embryos (Holstein-Japanese Red} Without Zona Pellucida
Boediono, Arief | M. Takagi | S. Saha | Suzuki, Tatsuyuki (2012)The objective of this study was to produce chimeric blastocysts by the aggregation of IVF bovine embryos (Holstein- Japanese Red}, without zonae pellucidae. The embryos were produced by the in vitro maturation and fertilization ... -
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
Saha, S. | M. Takagi | Boediono, Arief | Sumantri, Cece | Suzuki, Tatsuyuki (1996)In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ... -
Pregnancy rate and survival in culture of in vitro fertilized bovine embryos frozen in various cryoprotectants and thawed using a one-step system
Suzuki, Tatsuyuki | M. Takagi | M. Yamamoto | Saha. S. | Boediono, Arief | Ooe, Masato (1993)Bovine oocytes surrounded with compact cumulus cells were cultured for 20-22 h (38.5°C, 5 % C02) in modified TCM -199 supplemented with 5 % superovulated cow serum (SCS) and inseminated by in vitro capacitated sperm. Day ... -
Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
Suzuki, Tatsuyuki | M. Takagi | M. Yamamoto | Boediono, Arief | Saha, S. | H. Sakakibara | Ooe, Masato (1993)Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ... -
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
Boediono, Arief | M. Ooe | M. Yamamoto | M. Takagi | Saha, S. | Suzuki, Tatsuyuki (1993)Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ... -
Survival rate of frozan-thawed bovine IVF embryos in relation to exposure time using various cryoprotectans
M. Takagi | Boediono, Arief | Saha, S. | Suzuki, Tatsuyuki (1993)The objective of this study was to examine the relationship between cryoprotectant and equilibration t1me on development of IVF derived embryos in culture after thawing. Good to excellent quality day 1 to 8 blastocysts ...