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Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
(IPB (Bogor Agricultural University), 1993)
Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ...