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Influence of day-0 and day-7 superovulated cow serum during development of bovine oocytes in vitro
(Bogor Agriculture University, 1994)
Oocytes were matured in medium supplemented with 5% serum collected from superovulated cows at oestrus (Day-0 SCS) or at the time of embryo collection (Day-7 SCS), or in medium supplemented with fetal calf serum (FCS). ...
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(IPB (Bogor Agricultural University), 1996)
In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...
Direct rehydration of in vitro fertilised bovine embryos after vitrification
(1994)
VITRIFICATION is a valuable embryo freezing technique because it is simple, quick, economic (Rall and Fahy 1985), reli able and reasonably easy to apply under field conditions (Niemann 1991). ...
The Influence of Polyvinypyrrolidone on Freezing of Bovine IVF Blastocysts Following Biopsy
(IPB (Bogor Agricultural University), 1996)
A Study was conducted to develop a better freezing protocol for in vitro developed biosied blastocyst. Biopsied blastocysts exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different concentration (5,10, ...
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(IPB (Bogor Agricultural University), 1996)
The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ...
The influence of polyvinylpyrrolidone on freezing of bovine IVF blastocysts following biopsy
(IPB (Bogor Agricultural University), 1995)
A study was conducted to develop a better freezing protocol for in vitro developed biopsled bovine blastocysts. Biopsied blastocysts were exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different their in ...