Search
Now showing items 1-9 of 9
Chimera production in bovine and its sperm capacity
(Bogor Agriculture University, 1998)
In vitro produced bovine embryos were used to produce chimeras by combining the 8- cell stage embryos. Aggregated embryos were embedded in either 0, 1.0 and 1.2% agar and cultured in vitro. The aggregation ...
Chimeric blastocysts produced by aggregation between parthenogenetic and fertilized bovine embryos
(IPB (Bogor Agricultural University), 1996)
Chimeric blastocysts were produced by aggugation of parthenogenetic and fertilized embryos.The normal fertilized embryos were obtained by in vitro maturation, fertilization and culture procedures.To produce parthenogenetic ...
Development in vitro and in vivo of aggregated parthenogenetic bovine embryos
(IPB (Bogor Agricultural University), 2010)
Mature bovine oocytes were activated with 7% ethanol followed by cytochalasin B or D treatment. Most oocytes extruded a second polar body and formed one pronucleus when treated with 7% ethanol alone [35/43 (81%)]. With ...
Perkembangan In Vitro Embrio Sapi-Sapi Holstein dan Japanese Black
(IPB (Bogor Agriculture University), 1996)
Two experiments were conducted with 3,457 oocytes, aspirated from the ovaries of slaughtered Holstein and Japanese Black breeds cows to compare the use of superovulated cow serum (SCS) and fetal calf serum (FCS) supplementation ...
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(IPB (Bogor Agricultural University), 1996)
In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(IPB (Bogor Agricultural University), 1997)
The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...
The Influence of Polyvinypyrrolidone on Freezing of Bovine IVF Blastocysts Following Biopsy
(IPB (Bogor Agricultural University), 1996)
A Study was conducted to develop a better freezing protocol for in vitro developed biosied blastocyst. Biopsied blastocysts exposed to 1.8 M ethylene glycol (EG) + 0.05 M trehalose (T) and different concentration (5,10, ...
Fertilization and Development of Frozen–Thawed Germinal Vesicle Bovine Oocytes by a One-Step Dilution Method in Vitro
(IPB (Bogor Agricultural University), 1996)
The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen–thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown ...
Viability Of Bovine Blastocysts Obtained After 7, 8 Or 9 Days Of Culture Following Vitrification And One-Step Rehydration
(IPB (Bogor Agricultural University), 1993)
This study examined the morphological appearance, hatching rates and live:dead cell ratios following vitrification of in vitro produced (IVP) bovine blastocysts. Expanded blastocysts obtained after 7, 8 or 9 d of culture ...