Search
Now showing items 11-20 of 23
Offspring born from chimeras reconstructed from parthenogenetic and in vitro fertilized bovine embryos
(Bogor Agriculture University, 1999)
Chimeric embryos were produced by aggregation of parthenogenetic (Japanese Red breed) and in vitro fertilized (Holstein breed) bovine embryos at the Yamaguchi Research Station in Japan and by aggregation of parthenogenetic ...
Direct rehydration of in vitro fertilised bovine embryos after vitrification
(1994)
VITRIFICATION is a valuable embryo freezing technique because it is simple, quick, economic (Rall and Fahy 1985), reli able and reasonably easy to apply under field conditions (Niemann 1991). ...
Vitrification of bovine in vitro matured and pronuclear oocites with different vitrification solutions
(IPB (Bogor Agricultural University), 1993)
Vitrification of in vitro matured (IVM) and pronuclear (PN) oocytes was performed using three types of vitrification solutions (VS). IVM oocytes were vitrified with precooled (4°C) VS containing 40% ethylene glycol + 0.3M ...
Aggregation of parthenogenetic and fertilized embryos for producing chimera calves
(IPB (Bogor Agricultural University), 1993)
Chimeric blastocysts were produced by aggregation of parthenogenetic and fertilized demi-embryos from Japanese Brown breed and Holstein breed cattle, respectively. The fertilized embryos were obtained by in vitro maturation, ...
Development Of A Simple, Portable Carbon Dioxide Incubator For Production Of Bovine Ivf Embryos
(IPB (Bogor Agricultural University), 1993)
We have developed a simple, economical and portable C02 incubator for production of bovine IVF embryos in the field. The incubator consists of a metallic chamber (29L x 26W x 21 H em); the bottom plate of this chamber is ...
Production of chimeric calves by aggregation of in vitro-fertilized bovine embryos without zonae pellucidae
(IPB (Bogor Agricultural University), 1993)
Bovine embryos produced by in vitro maturation (IVM). fertilization (IVF) and culture (IVC) were used to produce aggregation chimeras. An aggregated chimera was produced by combining bovine IVF embryos (Holstein x Japanese ...
Pregnancy rate and survival in culture of in vitro fertilized bovine embryos frozen in various cryoprotectants and thawed using a one-step system
(IPB (Bogor Agricultural University), 1993)
Bovine oocytes surrounded with compact cumulus cells were cultured for 20-22 h (38.5°C, 5 % C02) in modified TCM -199 supplemented with 5 % superovulated cow serum (SCS) and inseminated by in vitro capacitated sperm. Day ...
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
(IPB (Bogor Agricultural University), 1996)
In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...
Pregnancy Rate And Survival In Culture Of In Vitro Fertilized Bovine Embryos Frozen In Various Cryoprotectants And Thawed Using A One-Step System
(IPB (Bogor Agricultural University), 1993)
Bovine oocytes surrounded with compact cumulus cells were cultured for 20 to 22 hours (38.5°C, 5% C02) in modified TCM-199 medium supplemented with 5% superovulated cow serum (SCS) and inseminated by in vitro capacitated ...
The effect of sperm-oocyte incubation time on in vitro embryo development using sperm from a tetraparental chimeric bull
(IPB (Bogor Agricultural University), 1997)
The present study was designed as 5 X 4 factorial to investigate the effects of using sperm from 5 bulls, and varied sperm-oocyte incubation times (5, 10, 15 and 20 h) on the fertilization, cleavage rates and blastocyst ...